Interferon Induced with Helicase C Domain 1 (IFIH1) antibody

Details for Product No. ABIN195468
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Antigen
Synonyms MDA5, Hlcd, IDDM19, MDA-5, RLR-2, 9130009C22Rik, Helicard, RGD1560496
Reactivity
Human
(61), (34), (1), (1), (1)
Host
Rabbit
(52), (10), (5)
Clonality
Polyclonal
Application
Western Blotting (WB)
(56), (40), (25), (18), (4), (3), (3), (2), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN195468
618.20 $
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Immunogen Synthetic peptide of part of N terminal of human MDA5 (TIEDRNRIAAAENNGN)
Isotype IgG
Purification Purified with antigen peptide
Alternative Name MDA5
Background A full-length cDNA encoding MDA5 was isolated by screening a melanoma differentiation-subtracted library and a placenta cDNA library, also known as Ifih1(Interferon induced with helicase C domain protein 1). Yoneyama et al. have identified a DExD/H box-containing RNA helicase, retinoic acid inducible gene I (RIG-I), as an essential component of the sensor of intracellular dsRNA (1). Furthermore, two other DexD/H-box-containing RNA helicases, MDA5 and LGP2, were found in the mammalian database (2). MDA5 acts as a positive regulator in the virus-induced activation of type I IFN genes. On the other hand, LGP2 functions as a dominant-negative regulator of RIG-I/MDA5-mediated signaling. These molecules have critical roles for antiviral innate immune responses.
Research Area Virology, Immunology
Application Notes Western blotting in concentration of 1 – 5?g/mL
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Interferon Induced with Helicase C Domain 1 (IFIH1) antibody lane 1: HeLa cell lysate without IFN beta stimulation. lane 2: HeLa cell lysate with IFN beta stimulation. When this antibody is used in Western Blotting, several bands appear although the antibody specifically detects the MDA5 protein. We recommend to perform positive and negative controls in parallel.
General Yoneyama, Kikuchi, Natsukawa et al.: "The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses." in: Nature immunology, Vol. 5, Issue 7, pp. 730-7, 2004 (PubMed).

Yoneyama, Kikuchi, Matsumoto et al.: "Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 2851-8, 2005 (PubMed).

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