Interferon Induced with Helicase C Domain 1 (IFIH1) antibody

Antigen: Interferon Induced with Helicase C Domain 1 (IFIH1)

Synonyms: Hlcd, MDA5, MDA-5, IDDM19, MGC133047

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN195468

Additional Information

Alternative name: MDA5

Immunogen: Synthetic peptide of part of N terminal of human MDA5 (TIEDRNRIAAAENNGN)

Format: Affinity-purified

Isotype: IgG

Description: A full-length cDNA encoding MDA5 was isolated by screening a melanoma differentiation-subtracted library and a placenta cDNA library, also known as Ifih1(Interferon induced with helicase C domain protein 1). Yoneyama et al. have identified a DExD/H box-containing RNA helicase, retinoic acid inducible gene I (RIG-I), as an essential component of the sensor of intracellular dsRNA (1). Furthermore, two other DexD/H-box-containing RNA helicases, MDA5 and LGP2, were found in the mammalian database (2). MDA5 acts as a positive regulator in the virus-induced activation of type I IFN genes. On the other hand, LGP2 functions as a dominant-negative regulator of RIG-I/MDA5-mediated signaling. These molecules have critical roles for antiviral innate immune responses.

Application Details

Application Notes: Western blotting in concentration of 1 – 5?g/mL

Purification: Purified with antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3

Storage: Lyophilized product, 5 years at 2 – 8 ?. Solution, 2 years at –20 ?. 1.0 mL deionized water will be added to the product (the conc. comes up 100ug/ml)

Research Area: Virology, Immunology

Restrictions: For Research Use only

Images

lane 1: HeLa cell lysate without IFN beta stimulation. lane 2: HeLa cell lysate with IFN beta stimulation. When this antibody is used in Western Blotting, several bands appear although the antibody specifically detects the MDA5 protein. We recommend to perform positive and negative controls in parallel.

Publications

Yoneyama, Kikuchi, Natsukawa et al.: "The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses." in: Nature immunology, Vol. 5, Issue 7, pp. 730-7, 2004 (PubMed).

Yoneyama, Kikuchi, Matsumoto et al.: "Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 2851-8, 2005 (PubMed).