Tuberous Sclerosis 1 (TSC1) (T1149) antibody

Antigen: Tuberous Sclerosis 1 (TSC1)

Synonyms: LAM, TSC, KIAA0243, MGC86987, hamartin, mKIAA0243, TSC1, MGC165718

Binding Site: T1149

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN195469

Additional Information

Alternative name: TSC1/Hamartin (T1149)

Immunogen: The synthetic peptide of a part of Human TSC1/Hamartin (GQLHIMDYNETHHEHS)

Format: Affinity-purified

Isotype: IgG

Description: TSC1 is a tumor-suppressor gene encoding a protein TSC1/Hamartin of about 130 kDa (ref. 1). Loss of its function causes TSC (tuberous sclerosis complex) or angiomyolipoma in human and in an animal model, TSC1 knockout mouse develops a renal tumor (ref. 2). TSC1/Hamartin, a product of TSC1, forms a complex with TSC2/Tuberin (ref. 3), a product of another tumor-suppressor gene TSC2 in cytoplasm (ref. 4). The complex suppresses mTOR activity and regulates cellular growth and division in a insulin signaling pathway (ref. 5). Under the condition of proliferation stimulus, forming of the complex is inhibited, and as a result, suppression to mTOR comes off and it leads to cellular growth and division.

Specificity: Crossreactivities with mouse

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1 - 5 ?g /mL, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 1 - 5 ?g /mL.

Purification: Purified with antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3

Storage: Lyophilized product, 5 years at 2 – 8 ?. Solution, 2 years at –20 ?. 1.0 mL deionized water will be added to the product (the conc. comes up 100ug/ml)

Research Area: Cancer, Diabetes

Restrictions: For Research Use only

Images

anti-Tuberous Sclerosis 1 (TSC1) (T1149) antibody

Human Pancreas

Publications

"Identification and characterization of the tuberous sclerosis gene on chromosome 16." in: Cell, Vol. 75, Issue 7, pp. 1305-15, 1994 (PubMed).

van Slegtenhorst, de Hoogt, Hermans et al.: "Identification of the tuberous sclerosis gene TSC1 on chromosome 9q34." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 805-8, 1997 (PubMed).

Plank, Yeung, Henske: "Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene, interacts with tuberin and appears to be localized to cytoplasmic vesicles." in: Cancer research, Vol. 58, Issue 21, pp. 4766-70, 1998 (PubMed).

Gao, Pan: "TSC1 and TSC2 tumor suppressors antagonize insulin signaling in cell growth." in: Genes & development, Vol. 15, Issue 11, pp. 1383-92, 2001 (PubMed).

Kobayashi, Minowa, Sugitani et al.: "A germ-line Tsc1 mutation causes tumor development and embryonic lethality that are similar, but not identical to, those caused by Tsc2 mutation in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 15, pp. 8762-7, 2001 (PubMed).