Alpha2, 6-Sialyltransferase antibody

Antigen: Alpha2, 6-Sialyltransferase

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN195493

Additional Information

Alternative name: alpha2, 6-Sialyltransferase (M2)

Immunogen: Synthetic peptide of a part of alpha2, 6-sialyltransferase (NSQLVTTEKRFLKDSL) (the part common to human, mouse and rat)

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: The histopathological picture of Alzheimer’s disease is characterized by senile plaques and neurofibrillary tangles, and because the senile plaques form first, they are considered the initial lesion. Senile plaques are known to be formed by accumulation of ?-amyloid peptide (A?). A? peptide is produced by the cleavage of amyloid precursor protein (APP) by two types of proteolytic enzymes. The first cleavage is performed by ?-secretase (BACE1), and the second ?-secretase. It is thought that their inhibitors may be capable of serving as safe drugs for the treatment of Alzheimer’s disease. In recent years a glycosyltransferase involved in the biosynthesis of sugar chains (?2, 6-sialyltransferase) has also been shown to be cleaved by BACE1. The cleavage site was identified at the same time, and as a result it was demonstrated that in rats it produces cleavaged-type ?2, 6-sialyltransferase (E41 Form). This product is purified antibody which can detect human, mouse or rat ?2, 6-sialyltransferase whether or not it is cleavaged.

Specificity: Reacts with Human, Mouse, Rat alpha2, 6-sialyltransferase

Application Details

Application Notes: Western blotting in concentration of 1 – 5ug/mL

Purification: Purified with antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3

Storage: Lyophilized product, 5 years at 2 – 8°C. Solution, 2 years at –20 °C. 1.0 mL deionized water will be added to the product (the conc. comes up 100ug/ml)

Research Area: Alzheimer's Disease

Restrictions: For Research Use only

Images

anti-Alpha2, 6-Sialyltransferase antibody

Publications

Publications: Kitazume-Kawaguchi, Dohmae, Takio et al.: "The relationship between ST6Gal I Golgi retention and its cleavage-secretion." in: Glycobiology, Vol. 9, Issue 12, pp. 1397-406, 2000 (PubMed).

Kitazume, Tachida, Oka et al.: "Alzheimer's beta-secretase, beta-site amyloid precursor protein-cleaving enzyme, is responsible for cleavage secretion of a Golgi-resident sialyltransferase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 24, pp. 13554-9, 2001 (PubMed).

Kitazume, Saido, Hashimoto: "[Identification of a novel substrate of Alzheimer's beta-secretase: beta-secretase dependent cleavage of alpha 2,6 sialyltransferase]" in: Seikagaku. The Journal of Japanese Biochemical Society, Vol. 74, Issue 9, pp. 1180-3, 2002 (PubMed).

Kitazume, Tachida, Oka et al.: "Characterization of alpha 2,6-sialyltransferase cleavage by Alzheimer's beta -secretase (BACE1)." in: The Journal of biological chemistry, Vol. 278, Issue 17, pp. 14865-71, 2003 (PubMed).