The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. This antibody detects endogenous levels of PKD/PKCμ only when phosphorylated at Serine 910.
Purification
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 910 (R-V-SP-I-L).
Verified
PRKD1
Reactivity: Human
WB, ELISA, IP
Host: Goat
Polyclonal
unconjugated
Application Notes
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.