AKT2 antibody (pSer474)

Catalog No. ABIN196826

Product Details anti-AKT2 Antibody

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Binding Specificity: pSer474

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2 / PKB beta (AKT2 Products)

Synonyms: AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody

Background: The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase

Gene ID: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling