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Retinoblastoma 1 antibody (pSer807)

RB1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN196841
  • Target See all Retinoblastoma 1 (RB1) Antibodies
    Retinoblastoma 1 (RB1)
    Binding Specificity
    • 32
    • 31
    • 29
    • 24
    • 24
    • 22
    • 20
    • 15
    • 11
    • 10
    • 10
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer807
    Reactivity
    • 292
    • 113
    • 95
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 253
    • 42
    Rabbit
    Clonality
    • 239
    • 56
    Polyclonal
    Conjugate
    • 148
    • 19
    • 17
    • 16
    • 11
    • 11
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Retinoblastoma 1 antibody is un-conjugated
    Application
    • 224
    • 132
    • 114
    • 80
    • 79
    • 42
    • 39
    • 36
    • 28
    • 19
    • 19
    • 9
    • 8
    • 6
    • 6
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Rb (phospho-Ser807) antibody detects endogenous levels of Rb only when phosphorylated at serine 807.
    Purification
    Immunoaffinity chromatography.
    Immunogen
    The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 807 (Y-I-SP-P-L).
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  • Application Notes
    Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    Retinoblastoma 1 (RB1)
    Alternative Name
    Retinoblastoma-Associated Protein / RB1 (RB1 Products)
    Synonyms
    OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
    Background
    Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
    Gene ID
    5925
    NCBI Accession
    NP_000312
    UniProt
    P06400
    Pathways
    Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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