Retinoblastoma 1 (RB1) (pSer780) antibody

Details for Product No. ABIN196842
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Antigen
Synonyms RB1, OSRC, RB, p105-Rb, pRb, pp110, Rb, Rb-1, zgc:154147, P105-RB, PP110, RB11
Epitope
pSer780
(36), (34), (31), (23), (19), (19), (19), (17), (17), (15), (13), (8), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(371), (228), (216), (109), (85), (65), (48), (7), (1), (1), (1)
Host
Rabbit
(331), (53), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(241), (112), (100), (93), (79), (48), (37), (31), (18), (11), (8), (8), (3), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN196842
357.50 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-SP-P-I).
Specificity This antibody detects endogenous levels of Rb only when phosphorylated at Serine 780.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Retinoblastoma-Associated Protein / RB1
Background Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
Gene ID 5925
NCBI Accession NP_000312
UniProt P06400
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
anti-Retinoblastoma 1 (RB1) (pSer780) antibody anti-Retinoblastoma 1 (RB1) (pSer780) antibody
anti-Retinoblastoma 1 (RB1) (pSer780) antibody (2) anti-Retinoblastoma 1 (RB1) (pSer780) antibody (Image 2)
anti-Retinoblastoma 1 (RB1) (pSer780) antibody (3) anti-Retinoblastoma 1 (RB1) (pSer780) antibody (Image 3)
Background publications Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).

Huang, Zegarra-Moro, Benson et al.: "Androgens repress Bcl-2 expression via activation of the retinoblastoma (RB) protein in prostate cancer cells." in: Oncogene, Vol. 23, Issue 12, pp. 2161-76, 2004 (PubMed).

Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Validation Images
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