Retinoblastoma 1 (RB1) (pSer780) antibody

Details for Product No. ABIN196843
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, P105-RB, PP110, RB11, zgc:154147, RB1
Epitope
pSer780
(32), (31), (29), (21), (20), (18), (17), (17), (16), (14), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(332), (217), (203), (109), (85), (70), (48), (1), (1), (1)
Host
Rabbit
(287), (56), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(209), (100), (93), (91), (84), (33), (24), (13), (10), (6), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN196843
Quantity 50 µg
Price
286.00 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-SP-P-I).
Specificity This antibody detects endogenous levels of Rb only when phosphorylated at Serine 780.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Retinoblastoma-associated protein / RB1
Background Alternate names: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
Gene ID 5925
NCBI Accession NP_000312.2
UniProt P06400
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-Retinoblastoma 1 (RB1) (pSer780) antibody anti-Retinoblastoma 1 (RB1) (pSer780) antibody
anti-Retinoblastoma 1 (RB1) (pSer780) antibody (2) anti-Retinoblastoma 1 (RB1) (pSer780) antibody (Image 2)
anti-Retinoblastoma 1 (RB1) (pSer780) antibody (3) anti-Retinoblastoma 1 (RB1) (pSer780) antibody (Image 3)
Background publications Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).

Huang, Zegarra-Moro, Benson et al.: "Androgens repress Bcl-2 expression via activation of the retinoblastoma (RB) protein in prostate cancer cells." in: Oncogene, Vol. 23, Issue 12, pp. 2161-76, 2004 (PubMed).

Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Hosts (287), (56), (2)
Reactivities (332), (217), (203), (109), (85), (70), (48), (1), (1), (1)
Applications (209), (100), (93), (91), (84), (33), (24), (13), (10), (6), (2), (1), (1)
Conjugates (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Epitopes (32), (31), (29), (21), (20), (18), (17), (17), (16), (14), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top