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LCK antibody (pTyr394)

LCK Reactivity: Human, Mouse WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN196860
  • Target See all LCK Antibodies
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Binding Specificity
    • 28
    • 16
    • 15
    • 11
    • 10
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr394
    Reactivity
    • 168
    • 95
    • 60
    • 9
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Human, Mouse
    Host
    • 152
    • 14
    • 2
    Rabbit
    Clonality
    • 147
    • 22
    Polyclonal
    Conjugate
    • 97
    • 10
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This LCK antibody is un-conjugated
    Application
    • 130
    • 88
    • 30
    • 29
    • 24
    • 20
    • 16
    • 14
    • 13
    • 10
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    This antibody detects endogenous levels of Lck only when phosphorylated at Tyrosine 394.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Mouse.
    Purification
    Immunoaffinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around the phosphorylation site of Tyrosine 394 (N-E-pY-T-A) derived from Human Lck.
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    Discover our top product LCK Primary Antibody
  • Application Notes
    Suitable for use in Western blot (1: 500approx. 1: 1000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Alternative Name
    LCK (LCK Products)
    Synonyms
    zgc:136695 antibody, LCK antibody, Hck-3 antibody, Lsk antibody, Lskt antibody, p56 antibody, p56Lck antibody, LSK antibody, YT16 antibody, p56lck antibody, pp58lck antibody, P56LCK antibody, tkl antibody, Lck1 antibody, Lcktkr antibody, LCK proto-oncogene, Src family tyrosine kinase antibody, lymphocyte protein tyrosine kinase antibody, lck antibody, LCK antibody, Lck antibody
    Background
    Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.Synonyms: LSK, Lymphocyte cell-specific protein-tyrosine kinase, Proto-oncogene tyrosine-protein kinase LCK, T cell-specific protein-tyrosine kinase, p56-LCK
    Molecular Weight
    56 kDa
    Gene ID
    3932
    NCBI Accession
    NP_001036236
    UniProt
    P06239
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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