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MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pSer189)

Details for Product anti-MAP2K3 Antibody No. ABIN196863, Supplier: Log in to see
Antigen
  • AW212142
  • map2k3
  • MAPKK3
  • MEK3
  • Mkk3
  • MKK3
  • mMKK3b
  • Prkmk3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • zMKK3
Alternatives
anti-Human MAP2K3 antibody for Western Blotting
Epitope
pSer189
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Reactivity
Human, Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Immunogen Synthetic phosphopeptide derived from human MKK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).
Specificity MKK3 (phospho-Ser189) antibody detects endogenous levels of MKK3 only when phosphorylated at serine189.
Purification Immunoaffinity chromatography.
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MEK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3
Gene ID 5606
NCBI Accession NP_002747
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pSer189) (ABIN196863) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (pSer189) antibody
 image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pSer189) (ABIN196863) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (pSer189) antibody (Image 2)
 image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pSer189) (ABIN196863) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (pSer189) antibody (Image 3)
Background publications Wang, Chen, Liao, Deng, Zhou, Huang, Sun: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002

Raingeaud, Whitmarsh, Barrett, Dérijard, Davis: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996