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CHEK2 antibody (pSer516)

CHEK2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN196867
  • Target See all CHEK2 Antibodies
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Binding Specificity
    • 30
    • 28
    • 21
    • 17
    • 16
    • 15
    • 15
    • 15
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer516
    Reactivity
    • 261
    • 96
    • 82
    • 16
    • 14
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 242
    • 20
    • 1
    • 1
    Rabbit
    Clonality
    • 228
    • 36
    Polyclonal
    Conjugate
    • 116
    • 14
    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    This CHEK2 antibody is un-conjugated
    Application
    • 206
    • 80
    • 79
    • 72
    • 37
    • 36
    • 33
    • 28
    • 28
    • 19
    • 18
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. Chk2 (phospho-Ser516) antibody detects endogenous levels of Chk2 only when phosphorylated at serine 516.
    Purification
    Immunoaffinity chromatography.
    Immunogen
    The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).
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    Discover our top product CHEK2 Primary Antibody
  • Application Notes
    Suitable for use in Western blot (1: 500approx. 1: 1000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Alternative Name
    CHK2 (CHEK2 Products)
    Synonyms
    CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody
    Background
    Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block. Defects in Chk2 are associated with Li-Fraumeni syndrome (LFS), a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in Chk2 are also found in some patients with prostate cancer (CaP) or osteosarcoma (OSRC). Substantial amino acid variants exist in some cancer tissues. Numerous truncated splice variants exist for this protein.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
    Gene ID
    11200
    NCBI Accession
    NP_001005735
    UniProt
    O96017
    Pathways
    p53 Signaling, Apoptosis, Cell Division Cycle
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