Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody

Details for Product No. ABIN196947
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Antigen
Synonyms
P27, ISG12, FAM14D, ISG12A, KIP1, MEN4, CDKN4, MEN1B, P27KIP1, AA408329, AI843786, Kip1, p27, p27Kip1, 1500011J20Rik, 2610202L11Rik, Bridge-1, Cdki1b, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18 ... show more
P27, ISG12, FAM14D, ISG12A, KIP1, MEN4, CDKN4, MEN1B, P27KIP1, AA408329, AI843786, Kip1, p27, p27Kip1, 1500011J20Rik, 2610202L11Rik, Bridge-1, Cdki1b, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B show less
Epitope
pThr187
(52), (43), (27), (27), (21), (20), (12), (9), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(345), (172), (147), (81), (61), (54), (36), (13), (6), (3), (3), (1), (1), (1), (1)
Host
Rabbit
(309), (65), (14), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(261), (129), (90), (60), (60), (56), (29), (21), (12), (10), (6), (5), (1)
Pubmed 4 references available
Catalog no. ABIN196947
Quantity 50 µg
Price
286.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).
Specificity P27Kip1 antibody detects endogenous levels of p27Kip1 only when phosphorylated at threonine 187.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name CDKN1B / KIP1
Background Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
Alternate names: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
Gene ID 1027
NCBI Accession NP_004055.1
UniProt P46527
Research Area Cancer, Cell Cycle
Application Notes Western Blot: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS((without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Supplier Images
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody
Background publications Ishida, Kitagawa, Hatakeyama et al.: "Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25146-54, 2000 (PubMed).

Fujita, Sato, Katayama et al.: "Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28706-13, 2002 (PubMed).

Connor, Kotchetkov, Cariou et al.: "CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 201-13, 2003 (PubMed).

Eguchi, Herschenhous, Kuzushita et al.: "Helicobacter pylori increases proteasome-mediated degradation of p27(kip1) in gastric epithelial cells." in: Cancer research, Vol. 63, Issue 15, pp. 4739-46, 2003 (PubMed).

Hosts (309), (65), (14), (1), (1)
Reactivities (345), (172), (147), (81), (61), (54), (36), (13), (6), (3), (3), (1), (1), (1), (1)
Applications (261), (129), (90), (60), (60), (56), (29), (21), (12), (10), (6), (5), (1)
Conjugates (13), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Epitopes (52), (43), (27), (27), (21), (20), (12), (9), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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