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Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody

Details for Product No. ABIN196979, Supplier: Log in to see
Antigen
  • G972R
  • HIRS-1
  • irs-1
  • IRS-1
  • irs1
  • IRS1
  • irs1-a
  • IRS1IRM
  • irsu
Alternatives
anti-Human Insulin Receptor Substrate 1 antibody for Enzyme Immunoassay
Epitope
pSer636
55
48
44
43
24
23
21
21
16
15
15
14
13
13
13
13
13
12
9
9
7
7
6
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
507
352
335
11
8
4
1
Host
Rabbit
504
30
Clonality
Polyclonal
Conjugate
Un-conjugated
13
11
11
11
10
10
10
10
10
10
10
10
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
308
213
137
110
102
79
30
29
12
4
2
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).
Specificity IRS-1 antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 636.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name IRS1 (IRS1 Antibody Abstract)
Background Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.Synonyms: IRS-1, Insulin receptor substrate 1
Gene ID 3667
NCBI Accession NP_005535
UniProt P35568
Research Area Cardiovascular, Atherosclerosis, Signaling, Growth Factors, Cancer
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50-1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody (ABIN196979) anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody
 image for anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody (ABIN196979) anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody (Image 2)
Background publications Tzatsos, Kandror: "Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 63-76, 2005

Ozes, Akca, Mayo, Gustin, Maehama, Dixon, Donner: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001

Kadowaki: "Insights into insulin resistance and type 2 diabetes from knockout mouse models." in: The Journal of clinical investigation, Vol. 106, Issue 4, pp. 459-65, 2000

Szanto, Kahn: "Selective interaction between leptin and insulin signaling pathways in a hepatic cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2355-60, 2000