You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibody

Details for Product No. ABIN196992, Supplier: Log in to see
Antigen
  • Bs
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • C-Kit
  • PBT
  • SCFR
  • MGF
  • c-kit
Alternatives
anti-Human Mast/stem Cell Growth Factor Receptor antibody for Immunofluorescence
Epitope
pTyr721
84
65
38
33
31
24
22
22
14
14
13
13
13
13
12
12
10
9
9
9
9
9
9
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
786
383
128
20
16
14
14
11
2
1
Host
Rabbit
618
218
204
4
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
105
88
81
69
48
40
15
14
11
11
11
8
8
8
8
8
7
6
5
5
4
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
362
337
334
174
129
104
92
58
38
30
19
12
11
11
8
4
4
3
2
2
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c- kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).
Specificity C-Kit antibody detects endogenous levels of c-kit only when phosphorylated at tyrosine 721.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name CD117 / C-Kit (KIT Antibody Abstract)
Background C-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
Gene ID 3815
NCBI Accession NP_000213
UniProt P10721
Research Area CD Antigens, Surface Receptors of Immune Cells, Growth Factors, Receptors, Proliferation Markers
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibody (ABIN196992) anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibody
Background publications Blume-Jensen, Jiang, Hyman et al.: "Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility." in: Nature genetics, Vol. 24, Issue 2, pp. 157-62, 2000 (PubMed).

Hirota, Isozaki, Moriyama et al.: "Gain-of-function mutations of c-kit in human gastrointestinal stromal tumors." in: Science (New York, N.Y.), Vol. 279, Issue 5350, pp. 577-80, 1998 (PubMed).

Sattler, Salgia, Shrikhande et al.: "Steel factor induces tyrosine phosphorylation of CRKL and binding of CRKL to a complex containing c-kit, phosphatidylinositol 3-kinase, and p120(CBL)." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10248-53, 1997 (PubMed).

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997 (PubMed).

Nocka, Tan, Chiu et al.: "Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W." in: The EMBO journal, Vol. 9, Issue 6, pp. 1805-13, 1990 (PubMed).