RPS6KB1 antibody (pSer411)

Catalog No. ABIN197034

Product Details anti-RPS6KB1 Antibody

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Binding Specificity: pSer411

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Serine 411.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 411 (I-R-SP-P-R).

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: RPS6KB1 / STK14A (RPS6KB1 Products)

Synonyms: PS6K antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, STK14A antibody, p70 S6KA antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, 10539 antibody, 7084 antibody, CG10539 antibody, DS6K antibody, Dmel\\CG10539 antibody, Dp70S6k antibody, Dp70[s6k] antibody, Dp70s6k antibody, S6k antibody, dS6K antibody, dS6k antibody, dp70[S6k] antibody, dp70s6k antibody, dps6k antibody, ds6k antibody, fs(3)07084 antibody, l(3)07084 antibody, p70 S6K antibody, p70/S6K antibody, p70S6K antibody, p70[S6 kinase] antibody, p70[S6K] antibody, p70[S6k] antibody, p70[S6kinase] antibody, p70s6K antibody, s6k antibody, s6k11 antibody, 2610318I15Rik antibody, 4732464A07Rik antibody, 70kDa antibody, AA959758 antibody, AI256796 antibody, AI314060 antibody, p70/85s6k antibody, p70s6k antibody, p70s6k-A antibody, p70-s6k antibody, ps6k antibody, rps6kb1 antibody, rps6kb1-A antibody, s6K1 antibody, stk14a antibody, fc51h01 antibody, wu:fc51h01 antibody, zgc:55713 antibody, ribosomal protein S6 kinase B1 antibody, Ribosomal protein S6 kinase antibody, ribosomal protein S6 kinase, polypeptide 1 antibody, ribosomal protein S6 kinase B1 L homeolog antibody, ribosomal protein S6 kinase B1 S homeolog antibody, ribosomal protein S6 kinase b, polypeptide 1b antibody, RPS6KB1 antibody, S6k antibody, Rps6kb1 antibody, rps6kb1.L antibody, rps6kb1.S antibody, rps6kb1b antibody

Background: Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1

Gene ID: 6198

NCBI Accession: NP_003152

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect