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EIF2AK2 antibody (pThr451)

EIF2AK2 Reactivity: Human IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN197059
  • Target See all EIF2AK2 Antibodies
    EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
    Binding Specificity
    • 46
    • 34
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr451
    Reactivity
    • 126
    • 74
    • 49
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 121
    • 10
    Rabbit
    Clonality
    • 107
    • 24
    Polyclonal
    Conjugate
    • 78
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This EIF2AK2 antibody is un-conjugated
    Application
    • 110
    • 46
    • 41
    • 40
    • 37
    • 31
    • 21
    • 17
    • 10
    • 10
    • 8
    • 4
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    PKR antibody detects endogenous levels of PKR only when phosphorylated at threonine 451.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-TP-L-R).
    Top Product
    Discover our top product EIF2AK2 Primary Antibody
  • Application Notes
    Immunohistochemistry: 1: 50approx. 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
    Alternative Name
    EIF2AK2 / PKR (EIF2AK2 Products)
    Synonyms
    PKR antibody, pkr antibody, EIF2AK2 antibody, DKFZp469K1934 antibody, EIF2AK1 antibody, PRKR antibody, 2310047A08Rik antibody, 4732414G15Rik antibody, AI467567 antibody, AI747578 antibody, Pkr antibody, Prkr antibody, Tik antibody, eukaryotic translation initiation factor 2 alpha kinase 2 antibody, eukaryotic translation initiation factor 2-alpha kinase 2 antibody, EIF2AK2 antibody, eif2ak2 antibody, Eif2ak2 antibody
    Background
    PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double-stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase
    Gene ID
    5610
    NCBI Accession
    NP_001129123
    UniProt
    P19525
    Pathways
    DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C
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