anti-GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (Ser21)

Details for Product anti-GSK3a Antibody No. ABIN197105
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Antigen
Synonyms GSK3A, CG5182, Dmel\\CG5182, GSK3a, 2700086H06Rik, xgsk3, GSK-3[a], GSK-3[b], GSK3, gsk3a
Epitope
Ser21
(60), (43), (28), (15), (12), (12), (10), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(260), (129), (98), (5), (3), (2), (2), (2), (2), (2)
Host
Rabbit
(216), (76), (4)
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
(20), (11), (11), (7), (7), (7), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(247), (130), (120), (43), (40), (34), (14), (12), (10), (7), (7), (5), (1)
Pubmed 5 references available
Quantity 50 μL
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Immunogen Synthetic non-phosphopeptide derived from human GSK3alpha around the phosphorylation site of serine 21 (T-S-SP-F-A).
Specificity GSK3alpha Antibody detects endogenous levels of total GSK3α protein.
Purification Affinity chromatography
Alternative Name GSK3 alpha (GSK3a Antibody Abstract)
Background Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily, GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97 % sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
Gene ID 2931
NCBI Accession NP_063937
UniProt P49840
Research Area Cardiovascular, Hypertrophy, Atherosclerosis, Wnt, Kinases/Phosphatases, Wnt Signaling
Pathways PI3K-Akt Signaling Pathway
Application Notes Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (2) anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (Image 2)
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (3) anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (Image 3)
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (4) anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibody (Image 4)
Background publications Welsh, Wilson, Proud: "GSK3: a SHAGGY frog story." in: Trends in cell biology, Vol. 6, Issue 7, pp. 274-9, 2004 (PubMed).

Barry, Graham, Fry et al.: "Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?" in: FEBS letters, Vol. 553, Issue 1-2, pp. 173-8, 2003 (PubMed).

Koivisto, Alavian, Hakkinen et al.: "Glycogen synthase kinase-3 regulates formation of long lamellipodia in human keratinocytes." in: Journal of cell science, Vol. 116, Issue Pt 18, pp. 3749-60, 2003 (PubMed).

Srivastava, Pandey: "Potential mechanism(s) involved in the regulation of glycogen synthesis by insulin." in: Molecular and cellular biochemistry, Vol. 182, Issue 1-2, pp. 135-41, 1999 (PubMed).

Cross, Alessi, Cohen et al.: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996 (PubMed).

Validation Images
Catalog No. ABIN197105
253.00 $
Plus shipping costs $45.00
Quantity
Price
50 μL
253.00 $

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