Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Thr394) antibody

Details for Product No. ABIN197107
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Antigen
Synonyms Prkmk2, CFC4, MAPKK2, MEK2, MKK2, PRKMK2, AA589381, MK2, ATMKK2, F27B13.50, F27B13_50, MAP KINASE KINASE 1, MAP kinase kinase 2, MK1
Epitope
Thr394
(41), (15), (12), (9), (8), (7), (7), (6), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(181), (94), (86), (13), (3), (2), (1)
Host
Rabbit
(152), (30), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(144), (60), (56), (37), (34), (30), (25), (13), (10), (8), (7), (4), (4), (1), (1)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN197107
253.00 $
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
Specificity MEK2 antibody detects endogenous levels of total MEK2 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name MAPKK 2
Background Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2
Gene ID 5605
NCBI Accession NP_109587
UniProt P36507
Research Area Kinases/Phosphatases
Application Notes Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Thr394) antibody anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Thr394) antibody
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Thr394) antibody (2) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Thr394) antibody (Image 2)
Background publications Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

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