STAT3 antibody (AA 725-729)

Catalog No. ABIN197177

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: AA 725-729

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of total STAT3 protein.

Purification: Affinity-Chromatography using epitope-specific peptide

Immunogen: Syntehtic peptide KLH conjugated corresponding to sequence around amino acids 725~729 (P-M-SP-P-R) derived from Human STAT3 protein.

Application Details

Application Notes: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Background: STAT3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. STAT3 is also constitutively activated in a number of human tumors, possesses oncogenic potential, and anti-apoptotic activities. Following phosphorylation at Y705, STAT3 dimerizes, translocates to the nucleus and binds DNA. Transcriptional activation seems to be regulated by phosphorylation at Ser 727. STAT3 exists as an alpha form (86kD) and a beta form (79kD) that lacks a 50 amino acid domain containing Ser 727.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3

Gene ID: 6774

NCBI Accession: NP_003141

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor