This antibody detects endogenous levels of total p95/NBS1 protein.
Purification
Affinity Chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human p95/NBS1 around the phosphorylation site of Serine 343 (S-L-SP-Q-G).
KO Validated
NBN
Reactivity: Human
WB, IF, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The p95 gene (also known as NBS1 and Nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95