Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN197259

Ezrin (EZR) (Tyr 353) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CVIL, CVL, VIL2, Vil2, AW146364, R75297, p81, ATVLN2, T11A7.16, T11A7_16, villin 2, ezr, vil2, wu:fc05c03, wu:fj31d06, cvil, cvl
Epitope
»Alternatives Tyr 353
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN197259
Quantity 50 µg
Price
242.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
Specificity Ezrin antibody detects endogenous levels of total Ezrin protein.
Purification Affinity-chromatography using epitope-specific immunogen
Alternative Name Ezrin
Background Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.
Alternate names: Cytovillin, EZR, VIL2, Villin-2, p81
Gene ID 7430
NCBI Accession NP_001104547.1
UniProt P15311
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes Western Blot: 1: 500~1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Crepaldi, Gautreau, Comoglio et al.: "Ezrin is an effector of hepatocyte growth factor-mediated migration and morphogenesis in epithelial cells." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 423-34, 1997 (PubMed).

Gautreau, Poullet, Louvard et al.: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, 1999 (PubMed).

Wang, Pfeiffer, Gaarde: "Activation of SRC tyrosine kinases in response to ICAM-1 ligation in pulmonary microvascular endothelial cells." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 47731-43, 2003 (PubMed).

Zhao, Shiue, Palkon et al.: "Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9485-90, 2004 (PubMed).

Alternatives for antigen "Ezrin (EZR)", type "Antibodies"
Hosts (194), (23), (11)
Reactivities (224), (161), (138), (52), (41), (36), (36), (12), (2), (2), (1)
Applications (177), (83), (50), (49), (26), (15), (8), (5), (4), (3), (1), (1), (1), (1)
Conjugates (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (29), (20), (18), (18), (16), (12), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon