Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody

Details for Product No. ABIN197296
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Antigen
Synonyms MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2, Prkmk3, mMKK3b, AW212142, Mkk3, map2k3, zMKK3
Epitope
Ser189
(41), (21), (12), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(185), (102), (92), (26), (25), (15), (12), (12), (2), (1), (1)
Host
Rabbit
(149), (39)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(155), (67), (48), (45), (38), (30), (12), (7), (3), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN197296
Quantity 0.1 mg
Price
308.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic non-phosphopeptide derived from human MKK3 around the phosphorylation site of serine189 (V-D-Sp-V-A).
Specificity MKK3 antibody AP02647PU detects endogenous levels of total MKK3 protein.
Purification Affinity chromatography
Alternative Name MAP2K3
Background MEK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Alternate names: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3
Gene ID 5606
NCBI Accession NP_002747.2
UniProt P46734
Research Area Kinases/Phosphatases
Application Notes Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody
anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody (2) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody (Image 2)
anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody (3) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (Ser189) antibody (Image 3)
Background publications Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).

Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Hosts (149), (39)
Reactivities (185), (102), (92), (26), (25), (15), (12), (12), (2), (1), (1)
Applications (155), (67), (48), (45), (38), (30), (12), (7), (3), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Epitopes (41), (21), (12), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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