MYL2 antibody (AA 16-20)

Catalog No. ABIN197362

Product Details anti-MYL2 Antibody

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Binding Specificity: AA 16-20

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody AP02680PU detects endogenous levels of total Myosin Light Chain 2 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around amino acids 16~20 (A-T-S-N-V) derived from Human Myosin Light Chain 2 Protein.

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Alternative Name: Myosin Light Chain 2 (MYL2) (MYL2 Products)

Synonyms: CMH10 antibody, MLC2 antibody, 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, MLC-2v antibody, Mlc2v antibody, Mylpc antibody, Mlc-2 antibody, myl2 antibody, zgc:136848 antibody, XMLC2 antibody, cmh10 antibody, mlc2 antibody, G2 antibody, MLC-2A antibody, MLC2V antibody, myosin light chain 2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin, light polypeptide 2, regulatory, cardiac, slow antibody, myosin, light chain 2b, regulatory, cardiac, slow antibody, myosin light chain 2 S homeolog antibody, myosin, light chain 2, regulatory, cardiac, slow antibody, MYL2 antibody, Mylpf antibody, Myl2 antibody, myl2b antibody, myl2.S antibody

Background: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.Synonyms: MLC-2, MLC-2v, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform

Gene ID: 4633

NCBI Accession: NP_000423

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction