Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN197386

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Tyr 493) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms SRK, STCD, STD, TZK, ZAP-70, Srk, zap70, MGC80670, MGC88962, ZAP70
Epitope
»Alternatives Tyr 493
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Catalog no. ABIN197386
Quantity 0.1 mg
Price
297.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-Yp-T-A).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Zap-70 antibody detects endogenous levels of total Zap-70 protein.
Purification Immunoaffinity chromatography.
Alternative Name ZAP70
Background Alternate names: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070.2
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Application Notes Suitable for use in Immunohistochemistry (1: 50-1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts (212), (101), (8), (4), (1)
Reactivities (322), (161), (107), (49), (48), (37), (24), (24), (1), (1), (1), (1), (1)
Applications (192), (104), (66), (63), (61), (40), (34), (23), (19), (6), (2), (2), (2), (1)
Conjugates (16), (12), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Epitopes (35), (33), (20), (19), (18), (14), (8), (8), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon