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PRKAA1 antibody (Thr174)

Name: PRKAA1 antibody (Thr174)
SKU: ABIN197410
Price: 555.6 USD
Availability: InStock

PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated

Image no. 2 for anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (Thr174) antibody (ABIN197410)

Image no. 1 for anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (Thr174) antibody (ABIN197410)

Image no. 3 for anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (Thr174) antibody (ABIN197410)

3 images

Catalog No. ABIN197410

Datasheet as PDF

Target See all PRKAA1 Antibodies

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
Binding Specificity
31

17

16

15

8

8

7

7

6

5

4

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Thr174
Reactivity
156

131

88

27

11

9

9

8

7

7

5

5

4

3

2

2

2

1

1

1

1
Human, Mouse, Rat
Host
156

17

1
Rabbit
Compatible Secondaries
Clonality
143

31
Polyclonal
Conjugate
86

10

9

9

5

5

5

5

5

5

5

4

4

4

4

4

3

1

1
This PRKAA1 antibody is un-conjugated
Application
137

58

45

41

40

39

34

19

13

12

11

3

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity

This antibody AP02704PU detects endogenous levels of total AMPK alpha-1/AMPK alpha-2 protein.

Purification

Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 alpha-1 around the phosphorylation site of threonine 174 (L-R-Tp-S-C).

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PRKAA1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 2B7 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr172), (pThr183) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Pig, Sheep WB, IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p), IF Host: Mouse Monoclonal 4D8 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 325-543), (Catalytic Subunit alpha) antibody
PRKAA1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer486) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 4D9 unconjugated

Application Notes

Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store the antibody (in aliquots) at -20 °C.
Target

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name

PRKAA1 (PRKAA1 Products)

Synonyms

AMPK antibody, AMPKa1 antibody, cb116 antibody, im:7154392 antibody, sb:cb130 antibody, wu:fa94c10 antibody, AMPK1 antibody, AMPKalpha1 antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, C130083N04Rik antibody, ampk antibody, ampka1 antibody, PRKAA1 antibody, protein kinase AMP-activated catalytic subunit alpha 1 antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antibody, PRKAA1 antibody, prkaa1 antibody, Prkaa1 antibody, prkaa1.L antibody

Background

Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2, AMPK alpha-1, AMPK alpha-2, AMPK1, AMPK2, PRKAA1, PRKAA2

Gene ID

5562

NCBI Accession

NP_006242

UniProt

Q13131

Pathways

AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect