Cortactin (CTTN) (Tyr 421) antibody

Details for Product No. ABIN197531
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Antigen
Synonyms Cttn, CTTN, cttn, ems1, CG3637, Dmel\CG3637, cortactin, 1110020L01Rik, Ems1, CTTN1, P85.25, EMS1, Cttnb
Epitope
Tyr 421
(37), (16), (11), (9), (8), (7), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(154), (94), (60), (14), (14), (14), (2), (1), (1), (1)
Host
Rabbit
(151), (17), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(142), (68), (64), (26), (20), (10), (6), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 50 µg
Shipping to United States (Change)
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Catalog No. ABIN197531
253.00 $
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Specificity Cortactin antibody detects endogenous levels of total Cortactin protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Cortactin
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Alternate names: Amplaxin, CTTN, EMS1, Oncogene EMS1
Gene ID 10090
UniProt Q60598
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Application Notes Immunohistochemistry: 1: 50-1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Supplier Images
anti-Cortactin (CTTN) (Tyr 421) antibody anti-Cortactin (CTTN) (Tyr 421) antibody
Background publications Huang, Liu, Haudenschild et al.: "The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 25770-6, 1998 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Head, Jiang, Li et al.: "Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton." in: Molecular biology of the cell, Vol. 14, Issue 8, pp. 3216-29, 2003 (PubMed).

Luo, Pan, Mines et al.: "CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis." in: The Journal of biological chemistry, Vol. 281, Issue 40, pp. 30081-93, 2006 (PubMed).

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