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Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3198-4127) antibody

Details for Product No. ABIN197617
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Antigen
Epitope
AA 3198-4127
(37), (15), (14), (14), (14), (11), (8), (7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(277), (121), (56), (40), (29), (13), (12), (4), (3), (1), (1), (1)
Host
Mouse
(220), (61), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(15), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(129), (82), (80), (74), (60), (48), (46), (20), (14), (9), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN197617
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Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells. Remarks: Epitope: aa 3198-4127
Clone 25-4
Isotype IgG2a
Characteristics Synonyms: DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit
Purification Protein A Chromatography.
Alternative Name DNA-PKcs / PRKDC / XRCC7
Background DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors,including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved inrepairing double strand DNA breaks.
Gene ID 5591
UniProt P78527
Application Notes Western Blotting: Use DNA-PKcs antibody at 2-4 µg/ml for 2hrs at RT. Gel Supershift (Not optimum)Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A) (Ab 2µg/mg protein lysate. Positive Control: LS174T cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or in aliquots at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3198-4127) antibody anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3198-4127) antibody
Background publications Carter, Vancurová, Sun et al.: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).

Poltoratsky, Shi, York et al.: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Kirchgessner, Patil, Evans et al.: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Konno-Sato, Wu, Carter: "Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK)." in: Biochemistry and molecular biology international, Vol. 31, Issue 1, pp. 113-24, 1994 (PubMed).

Han, Malik, Carter et al.: "DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25035-40, 1996 (PubMed).

Validation Images
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