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Details for Product No. ABIN197617

Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3198-4127) antibody

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Antigen
Synonyms HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, dna-pkcs, dnapk, dnpk1, hyrc, hyrc1, prkdc-A, xrcc7, AI326420, AU019811, DNAPDcs, DOXNPH, dxnph, p460, scid, slip, DNA-PKCS, PRKDC
Epitope
»Alternatives AA 3198-4127
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN197617
Quantity 0.1 mg
Price
643.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells. Remarks: Epitope: aa 3198-4127
Clone 25-4
Isotype IgG2a
Characteristics Synonyms: DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit
Purification Protein A Chromatography.
Alternative Name DNA-PKcs / PRKDC / XRCC7
Background DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors,including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved inrepairing double strand DNA breaks.
Gene ID 5591
UniProt P78527
Application Notes Western Blotting: Use DNA-PKcs antibody at 2-4 µg/ml for 2hrs at RT. Gel Supershift (Not optimum)Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A) (Ab 2µg/mg protein lysate. Positive Control: LS174T cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or in aliquots at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Carter, Vancurová, Sun et al.: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).

Poltoratsky, Shi, York et al.: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Kirchgessner, Patil, Evans et al.: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Konno-Sato, Wu, Carter: "Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK)." in: Biochemistry and molecular biology international, Vol. 31, Issue 1, pp. 113-24, 1994 (PubMed).

Han, Malik, Carter et al.: "DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25035-40, 1996 (PubMed).

Alternatives for antigen "Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC)", type "Antibodies"
Hosts (175), (61)
Reactivities (230), (109), (46), (41), (30), (12), (12), (6), (4), (1), (1)
Applications (125), (86), (70), (63), (54), (46), (17), (15), (14), (3), (2), (2), (1), (1), (1)
Conjugates (10), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7)
Epitopes (38), (14), (13), (13), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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