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PRKDC antibody (Protein Kinase, DNA-Activated, Catalytic Polypeptide) (AA 3198-4127)

Details for Product anti-PRKDC Antibody No. ABIN197617, Supplier: Log in to see
Antigen
  • AI326420
  • AU019811
  • DNA-PKCS
  • DNA-PKcs
  • dna-pkcs
  • DNAPDcs
  • dnapk
  • DNAPK
  • dnpk1
  • DNPK1
  • DOXNPH
  • dxnph
  • HYRC
  • hyrc
  • HYRC1
  • hyrc1
  • p350
  • p460
  • PRKDC
  • prkdc-A
  • scid
  • slip
  • XRCC7
  • xrcc7
Alternatives
anti-Human PRKDC antibody for Electrophoretic Mobility-Shift Assay
Epitope
AA 3198-4127
48
22
18
17
16
16
15
14
11
8
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
399
131
42
5
4
3
3
3
2
1
1
1
Host
Mouse
305
99
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This PRKDC antibody is un-conjugated
19
18
17
12
9
8
8
8
8
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
192
137
106
95
83
77
50
42
16
8
8
6
3
3
2
2
1
1
Supplier
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Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells. Remarks: Epitope: aa 3198-4127
Clone 25-04-14
Isotype IgG2a
Specificity This DNA-PKcs antibody reacts with only the intact DNA-PKcs protein and does not detect its degradation products.
Cellular Localization: Nuclear.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit
Purification Protein A Chromatography.
Alternative Name DNA-PKcs / PRKDC / XRCC7 (PRKDC Antibody Abstract)
Background DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
Gene ID 5591
UniProt P78527
Pathways DNA Damage Repair
Application Notes Western Blotting: Use DNA-PKcs antibody at 2-4 μg/mL for 2hrs at RT. Gel Supershift (Not optimum)Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A) (Ab 2 μg/mg protein lysate. Positive Control: LS174T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-PRKDC antibody (Protein Kinase, DNA-Activated, Catalytic Polypeptide) (AA 3198-4127) (ABIN197617) anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3198-4127) antibody
Background publications Han, Malik, Carter, Reeves, Wyche, Hendrickson: "DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25035-40, 1996 (PubMed).

Poltoratsky, Shi, York, Lieber, Carter: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Kirchgessner, Patil, Evans, Cuomo, Fried, Carter, Oettinger, Brown: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Konno-Sato, Wu, Carter: "Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK)." in: Biochemistry and molecular biology international, Vol. 31, Issue 1, pp. 113-24, 1994 (PubMed).

Carter, Vancurová, Sun, Lou, DeLeon: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).