Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody

Details for Product No. ABIN197619
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Antigen
Synonyms HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, dna-pkcs, dnapk, dnpk1, hyrc, hyrc1, prkdc-A, xrcc7, AI326420, AU019811, DNAPDcs, DOXNPH, dxnph, p460, scid, slip, DNA-PKCS, PRKDC
Reactivity
Human
(225), (109), (46), (41), (30), (12), (12), (5), (4), (1), (1)
Host
Mouse
(171), (61)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(127), (77), (70), (63), (50), (45), (21), (20), (14), (3), (2), (2), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN197619
Quantity 0.1 mg
Price
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Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells (see ref. 1)
Clone 42-26
Isotype IgG1
Characteristics Synonyms: DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit
Purification Protein G chomatography
Alternative Name DNA-PKcs / PRKDC / XRCC7
Background DNA-PKcs phosphorylates certain transcription factors, including Sp 1, Oct-1, p53, andSV40 large T antigen in vitro. In vivo, it is involved in reparing double strand DNA breaks.
UniProt P78527
Application Notes Immunofluorescence. Immunohistochemistry on formalin-fixed paraffin embedded sections: 1/400, 20 min at RT. (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,pH 6.0, for 10-20 min followed by cooling at RT for 20 min, and use of a high-sensitivitydetection system). Recommended positive control: Testis. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Carter, Vancurová, Sun et al.: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).

Poltoratsky, Shi, York et al.: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Kirchgessner, Patil, Evans et al.: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Konno-Sato, Wu, Carter: "Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK)." in: Biochemistry and molecular biology international, Vol. 31, Issue 1, pp. 113-24, 1994 (PubMed).

Han, Malik, Carter et al.: "DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25035-40, 1996 (PubMed).

Hosts (171), (61)
Reactivities (225), (109), (46), (41), (30), (12), (12), (5), (4), (1), (1)
Applications (127), (77), (70), (63), (50), (45), (21), (20), (14), (3), (2), (2), (2), (1), (1)
Conjugates (10), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7)
Epitopes (40), (14), (13), (13), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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