Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody

Details for Product No. ABIN197619
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Antigen
Reactivity
Human
(273), (107), (42), (29), (28), (13), (4), (3), (1), (1)
Host
Mouse
(216), (60), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (4), (4), (4)
Application
Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(133), (80), (70), (69), (64), (46), (46), (18), (13), (9), (9), (8), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN197619
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Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells (see ref. 1)
Clone 42-26
Isotype IgG1
Specificity The antibody recognizes the intact DNA-PKcs as well as its degraded products. Cellular localization: Nuclear. Molecular Weight of Antigen: 460 kDa. Epitope: aa 2713-3198.
Characteristics Synonyms: DNPK1, p460, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit
Purification Protein G chomatography
Alternative Name DNA-PKcs / PRKDC / XRCC7
Background DNA-PKcs phosphorylates certain transcription factors, including Sp 1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in reparing double strand DNA breaks.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
Gene ID 5591
UniProt P78527
Application Notes Immunofluorescence. Immunohistochemistry on formalin-fixed paraffin embedded sections: 1/400, 20 min at RT. (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, and use of a high-sensitivitydetection system). Recommended positive control: Testis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, containing 0.2 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Carter, Vancurová, Sun et al.: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, 1991 (PubMed).

Poltoratsky, Shi, York et al.: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, 1995 (PubMed).

Kirchgessner, Patil, Evans et al.: "DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect." in: Science (New York, N.Y.), Vol. 267, Issue 5201, pp. 1178-83, 1995 (PubMed).

Konno-Sato, Wu, Carter: "Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK)." in: Biochemistry and molecular biology international, Vol. 31, Issue 1, pp. 113-24, 1994 (PubMed).

Han, Malik, Carter et al.: "DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25035-40, 1996 (PubMed).

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