Phosphotyrosine (phosphorylated) antibody (HRP)

Details for Product No. ABIN197634
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Antigen
Epitope
phosphorylated
(48), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Amino Acid
(151), (51), (46), (20), (20), (16), (7), (3), (1)
Host
Mouse
(200), (62), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
(27), (23), (22), (16), (9), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA)
(197), (118), (100), (68), (66), (52), (32), (24), (15), (12), (10), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.5 mL
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Immunogen U266 cell line.
Clone PY20
Isotype IgG2b
Purification Affinity chromatography
Target Type Amino Acid
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes ELISA: 1/1000 - 1/4000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 50 % PBS, pH 7.4 and 50 % Glycerol
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Ruff-Jamison, Campos-González, Glenney: "Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features." in: The Journal of biological chemistry, Vol. 266, Issue 10, pp. 6607-13, 1991 (PubMed).

Validation Images
Catalog No. ABIN197634
308.00 $
Plus shipping costs $45.00
Quantity
Price
0.5 mL
308.00 $

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