CD5 Molecule (CD5) antibody

Antigen: CD5 Molecule (CD5)

Synonyms: T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790

Clonality: Monoclonal (BV1)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197677

Additional Information

Alternative name: CD5

Immunogen: A synthetic cyclic peptides from the antigen

Format: Concentrated

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: BV1

Specificity: CD5 is a glycoprotein monomer with a MW of 67 000 belonging to the scavenger receptor cysteine-rich (SRCR) family of extracellular domain-like structures, and it possesses a large cytoplasmic domain suitable for signal transduction. CD5 is a T-cell marker that is also reacts with a range of neoplastic B-cells, e.g. B-cell chronic lymphocytic leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), and mantle cell lymphoma. CD5 is expressed in T lymphocyte subsets and is modulated during cellular activation, however it does not react with granulocytes or monocytes.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as cell culture supernatant and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Kasaian, Ikematsu, Casali: "CD5+ B lymphocytes." in: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), Vol. 197, Issue 3, pp. 226-41, 1991 (PubMed).

Jones, Clabby, Dialynas et al.: "Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1." in: Nature, Vol. 323, Issue 6086, pp. 346-9, 1986 (PubMed).

Chan, Banks, Cleary et al.: "A proposal for classification of lymphoid neoplasms (by the International Lymphoma Study Group)." in: Histopathology, Vol. 25, Issue 6, pp. 517-36, 1995 (PubMed).