CD163 Molecule (CD163) antibody

Antigen: CD163 Molecule (CD163)

Synonyms: CD163, DMBT1, M130, MM130, CD163v2, CD163v3

Clonality: Monoclonal (10D6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197678

Additional Information

Alternative name: CD163

Immunogen: Human CD163 antigen.

Format: Concentrated

Isotype: IgG1  (Matching secondary antibodies)

Clone: 10D6

Specificity: The CD163 molecule is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 protein is restricted in its expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhans cells. In addition, multinucleated cells within inflammatory lesions are reported not to express CD163 protein. The protein is upregulated by glucocorticoids and downregulated by the immunosuppressant cyclosporin A and by phorbol esters.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Hoegger, Dreier, Droste et al.: "Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 4, pp. 1883-90, 1998 (PubMed).

Droste, Sorg, Hoegger: "Shedding of CD163, a novel regulatory mechanism for a member of the scavenger receptor cysteine-rich family." in: Biochemical and biophysical research communications, Vol. 256, Issue 1, pp. 110-3, 1999 (PubMed).