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Epidermal Growth Factor Receptor (EGFR) antibody

Antigen

Epidermal Growth Factor Receptor (EGFR)
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33,  ... show more
Clonality Monoclonal (31G7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN197682
Quantity 1.0 ml  (100ug/ml approximately)  (Variants)
Price 327.00 $   Plus shipping costs $35.00
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Additional Information
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Immunogen Human EGFR derived from A-431 cell line.
Format Concentrated
Isotype IgG1  (Matching secondary antibodies)
Clone 31G7
Specificity The epidermal growth factor receptor (EGFR) is a 170 kD transmembrane protein consisting of an extracellular EGF-binding domain, a short transmembrane region and an intracellular domain with ligand-activated tyrosine kinase activity. EGFR is expressed in many normal epithelial tissues, particularly in the basal layers of stratified epithelium and in squamous epithelium. Over expression of EGFR has been observed in many types of neoplasia, as a result of gene amplification and/or increased protein transcription.
Synonyms ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Membrane, cytoplasm
Concentration 100ug/ml approximately
Buffer This antibody is supplied as diluted ascites containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Secondary Antibodies Variants back to top

Publications Rusch, Klimstra, Venkatraman: "Molecular markers help characterize neuroendocrine lung tumors." in: The Annals of thoracic surgery, Vol. 62, Issue 3, pp. 798-809; discussion 809-10, 1996 (PubMed).

Iwakura, Miwa, Sakai et al.: "Human hypertrophic nonunion tissue contains mesenchymal progenitor cells with multilineage capacity in vitro." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 27, Issue 2, pp. 208-15, 2009 (PubMed).

Alternatives
Application Details Publications Secondary Antibodies Variants back to top

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts Rabbit (418), Mouse (166), Rat (8), Sheep (7), Goat (2)
Reactivities Human (580), Mouse (Murine) (251), Rat (Rattus) (227), Dog (Canine) (33), Rabbit (18), Pig (Porcine) (17), Cow (Bovine) (6), Chicken (5), Horse (Equine) (2), Sheep (Ovine) (2)
Applications Western Blotting (WB) (418), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (134), Immunoprecipitation (IP) (120), Enzyme Immunoassay (EIA) (113), Immunohistochemistry (IHC) (102), Immunofluorescence (IF) (98), ELISA (92), Flow Cytometry (FACS) (90), Immunohistochemistry (Frozen Sections) (IHC (fro)) (43), Dot Blot (Dot) (28), Blocking Antibody (Inhibition) (16), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2), Blocking Antibody (Inhibition) (1)
Conjugates Biotin (35), FITC (5), Alexa Fluor 488 (3), PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes pTyr1197 (19), C-Term (16), pTyr869 (15), pTyr1092 (14), pTyr1172 (13), pThr678 (12), pTyr1110 (12), pSer1070 (11), pThr693 (10), N-Term (5), pTyr1016 (4), pTyr1069 (4), AA 1-203 (2), AA 1140-1160 (2), AA 960-980 (2), Extracell. non Ligand binding Site (2), S695 (2), Y1125 (2), Y869 (2), Y998 (2), pSer1026 (2), pSer1071 (2), pSer1166 (2), pSer1190 (2), pSer695 (2), pSer768 (2), pTyr1125 (2), pTyr1173 (2), pTyr998 (2), AA 1189-1199 (1), AA 985-996 (1), Ab-1016 (1), Ab-1026 (1), Ab-1069 (1), Ab-1071 (1), Amino Acids 1165-1186 (1), External Domain (1), Extracellular non-ligand binding site (1), S1026 (1), S1070 (1), S1071 (1), S1166 (1), S1190 (1), S991 (1), T678 (1), TYR1173 (1), Y1016 (1), Y1069 (1), Y1092 (1), Y1110 (1), Y1172 (1), Y978 (1), extracellular ligand binding site (1), internal (1), pS1070 (1), pS768 (1), pSer1142 (1), pSer967 (1), pTyr1101 (1), pTyr1173, pTyr1248 (1), pTyr992 (1), pY1016 (1), pY1092 (1), pY1172 (1), prylated At Threonine 654 (1), prylated At Tyrosine 1045 (1), prylated At Tyrosine 1173 (1), prylated At Tyrosine 845 (1), prylated at Serine 1047 (1)