Paired Box 5 (PAX5) antibody

Antigen: Paired Box 5 (PAX5)

Synonyms: BSAP, PAX5, KLP, EBB-1, Pax-5, noi, pax2a, pax2.1, paxzf-b, Pax[Zf-b]

Clonality: Monoclonal (BV6)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197689

Additional Information

Alternative name: Pax-5

Immunogen: A synthetic cyclic peptides from the antigen

Format: Concentrated

Isotype: IgG

Clone: BV6

Specificity: The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. PAX-5 expression is not only continuously required for B cell lineage commitment during early B cell development but also for B lineage maintenance. PAX-5 is found in most cases of mature and precursor B-cell non-Hodgkin lymphomas/leukemias. Pax-5 is not detected in Multiple myeloma and solitary plasmacytoma, making it useful for such differentiation. Diffuse large B-cell lymphomas do express Pax-5 except for those with terminal B-cell differentiation. T-cell neoplasms do not stain with anti-Pax-5, however there is a strong association with CD20 expression.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50 to 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Purification: Concentrated

Buffer: This antibody is supplied as cell culture supernatant and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Willenbrock, Ichinohasama, Kadin et al.: "T-cell variant of classical Hodgkin's lymphoma with nodal and cutaneous manifestations demonstrated by single-cell polymerase chain reaction." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 9, pp. 1103-9, 2002 (PubMed).

Torlakovic, Torlakovic, Nguyen et al.: "The value of anti-pax-5 immunostaining in routinely fixed and paraffin-embedded sections: a novel pan pre-B and B-cell marker." in: The American journal of surgical pathology, Vol. 26, Issue 10, pp. 1343-50, 2002 (PubMed).