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alpha-Fetoprotein (AFP) antibody

Antigen

alpha-Fetoprotein (AFP)
Synonyms FETA, HPAFP, AFP, MGC128267
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN197693
Quantity 1.0 ml  (100ug/ml approximately)  (Variants)
Price 117.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Publications Alternatives Secondary Antibodies Variants back to top

Alternative name Alpha-1-Fetoprotein (AFP)
Immunogen Alpha-1-fetoprotein isolated from human cord serum.
Format Concentrated
Specificity This antibody reacts with alpha-1-fetoprotein. A positive staining in the hepatocytes of fetal tissue was observed. This antibody does not react with any other cell or tissue types.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Fetal liver. Cellular localization: Cytoplasmic
Concentration 100ug/ml approximately
Buffer Purified rabbit antiserum against human AFP containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
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Publications MacDonald, Kelly: "The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 87, Issue 3, pp. 367-72, 1978 (PubMed).

Alternatives
Application Details Publications Secondary Antibodies Variants back to top

Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
Hosts Mouse (146), Rabbit (82), Goat (14), Sheep (3), Duck (2)
Reactivities Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
Applications Western Blotting (WB) (83), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (55), ELISA (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (20), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
Conjugates HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
Epitopes N-Term (3), a (2), Center (1), b (1), c (1)