Caudal Type Homeobox 1 (CDX1) antibody

Antigen: Caudal Type Homeobox 1 (CDX1)

Synonyms: MGC116915, Cdx, Cdx-1, cdx-1, xcad2, xtcad2, CDXA, CDX-A, CHox-cad, CDX1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197697

Additional Information

Alternative name: Cdx1

Immunogen: A synthetic peptide corresponding to human Cdx-1 and Cdx2.

Format: Concentrated

Specificity: The antibody specifically recognizes homeodomain proteins Cdx1 and Cdx2 (28 kDa and 33 kDa, respectively). The antibody recognizes human and rodent Cdx1 and Cdx2. Other species reactivity has not been confirmed. Cdx1 (Caudal-type homeobox protein 1) and Cdx2 (Caudal-type homeobox protein 2, CDX-2) are members of the caudal homeobox family of transcription factors, containing one homeobox domain. Cdx1 and Cdx2 are involved in the transcriptional regulation of multiple genes expressed in intestinal epithelium with functions ranging from early differentiation to maintenance of the intestinal lining.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Intestine. Cellular localization: Cytoplasmic, Nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Cdx1 protein containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union