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Antibodies
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anti-Hepatitis B Core Antigen (HBcAg) antibody
Hepatitis B Core Antigen (HBcAg) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Enzyme Immunoassay (EIA) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Western Blotting (WB) (21), Immunoprecipitation (IP) (19), ELISA (8), Flow Cytometry (FACS) (8), Immunofluorescence (IF) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), ELISA (Detection) (4), ELISA (Capture) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN197704 |
| Quantity |
0.5 ml (100ug/ml approximately) (Variants) |
| Price |
101.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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HBcAg purified from the lysates of E. coli infected with the viral core DNA.
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Format
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Concentrated
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Specificity
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This antigen reacts in the nuclei of the liver cells of the Hepatitis type B virus infected patients. However, occasional staining may also be seen in the perinuclear cytoplasm of the infected cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Infected liver. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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Whole rabbit antiserum containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Riedl, Wieland, Lamberth et al.: "Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 370-80, 2009 (PubMed).
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Alternatives for antigen "Hepatitis B Core Antigen (HBcAg)", type "Antibodies"
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Hosts
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Mouse (40), Rabbit (8)
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Reactivities
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Hepatitis B (HBV) (8), Human (4)
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Applications
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Enzyme Immunoassay (EIA) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Western Blotting (WB) (21), Immunoprecipitation (IP) (19), ELISA (8), Flow Cytometry (FACS) (8), Immunofluorescence (IF) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), ELISA (Detection) (4), ELISA (Capture) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Biotin (2), HRP (1)
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Epitopes
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ayw (5), AA 1-10 N-Term End (1), ayw AA 130-140 (1), ayw AA 138-145 (1)
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Alternatives 
