CD25 antibody
| Antigen |
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| Clonality |
Monoclonal (IL2R-1) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN197714 |
| Quantity |
6 ml (1 to 4 ug/ml) (Variants) |
| Price |
169.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD25/IL-2R alpha
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Immunogen
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BALB/C mice were injected with recombinant human interleukin- 2 receptor.
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Format
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Liquid (prediluted)
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Clone
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IL2R-1
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Specificity
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This antibody is specific to a 55 kD protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "CD25", type "Antibodies"
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Hosts
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Rat (46), Mouse (34)
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Reactivities
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Mouse (Murine) (45), Human (25), Rat (Rattus) (6), Cow (Bovine) (3), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (76), Immunofluorescence (IF) (9), Immunoprecipitation (IP) (9), Blocking Antibody (Inhibition) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Functional Studies (Func) (2), Immunohistochemistry (IHC) (2), Western Blotting (WB) (2), Blocking Antibody (Inhibition) (1), ELISA (1)
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Conjugates
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FITC (13), PE (11), Biotin (10), APC (5), PE,Cy7 (4), RPE (4), SPRD (4), Alexa Fluor 488 (1), Alexa Fluor 700 (1), PE-Dyomics 647 (1), Pacific Blue (1), RPE,Cy5 (1)
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Alternatives 
