Cyclin E Protein antibody

Antigen: Cyclin E Protein

Clonality: Monoclonal (HE12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197716

Additional Information

Immunogen: Synthetic peptide corresponding to human cyclin E protein.

Format: Liquid (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: HE12

Specificity: This antibody recognizes a doublet of 50 kD and a single band of 42 kD identified as cyclin E. Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Duli?, Lees, Reed: "Association of human cyclin E with a periodic G1-S phase protein kinase." in: Science (New York, N.Y.), Vol. 257, Issue 5078, pp. 1958-61, 1992 (PubMed).

Koff, Cross, Fisher et al.: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991 (PubMed).

Ohtsubo, Theodoras, Schumacher et al.: "Human cyclin E, a nuclear protein essential for the G1-to-S phase transition." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2612-24, 1995 (PubMed).

Sewing, Roenicke, Buerger et al.: "Alternative splicing of human cyclin E." in: Journal of cell science, Vol. 107 ( Pt 2), pp. 581-8, 1994 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).