Cytokeratin 5 (KRT5) antibody

Antigen: Cytokeratin 5 (KRT5)

Synonyms: KRT79, CK5, KRT5, K5, Tfip8, Krt2-5, AW146334, 3300001P10Rik, DDD, EBS2, KRT5A

Clonality: Monoclonal (XM26)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197717

Additional Information

Alternative name: Cytokeratin 5

Immunogen: BALB/C mice were injected with prokaryotic recombinant protein corresponding to 103 amino acid portion of C-terminal region of the human cytokeratin 5 molecule.

Format: Liquid (prediluted)

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: XM26

Specificity: This antibody reacts with cytokeratin 5 intermediate filament protein. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kD protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Irvine, McKenna, Jenkinson et al.: "A mutation in the V1 domain of keratin 5 causes epidermolysis bullosa simplex with mottled pigmentation." in: The Journal of investigative dermatology, Vol. 108, Issue 5, pp. 809-10, 1997 (PubMed).

Whittock, Eady, McGrath: "Genomic organization and amplification of the human epidermal type II keratin genes K1 and K5." in: Biochemical and biophysical research communications, Vol. 274, Issue 1, pp. 149-52, 2000 (PubMed).