Keratin 14 (KRT14) antibody
| Antigen |
|
| Synonyms |
K14, NFJ, CK14, EBS3, EBS4, Krt1-14, AI626930, Krt-1.14, Krt14l, Krt14, Ka14, KRT14, ouro2 |
| Clonality |
Monoclonal (LL002) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
Western Blotting (WB) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (IHC) (12), Flow Cytometry (FACS) (11), Immunofluorescence (IF) (6), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunoprecipitation (IP) (1)
|
| Catalog no. |
ABIN197719 |
| Quantity |
6 ml (1 to 4 ug/ml) (Variants) |
| Price |
181.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Cytokeratin 14
|
|
Immunogen
|
A synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14.
|
|
Format
|
Liquid (prediluted)
|
|
Clone
|
LL002
|
|
Description
|
Keratin 14 is a member of the keratin family, the most diverse group of intermediatefilaments. It is usually found as a heterotetramer with two molecules of keratin 5, a typeII keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genesfor these keratins are associated with epidermolysis bullosa simplex. Keratin 14 hasbeen studied as a prognostic marker in breast cancer. This antibody labels the basallayer of stratifying squamous and non-squamous epithelia. The staining patterniscytoplasmic. It recognizes basal cell carcinomas and squamous cell carcinomas. Alternate Names: anti-CK 14 antibody, anti-ck14 antibody, anti-Cytokeratin 14 antibody,anti-Cytokeratin14 antibody,anti-Dowling Meara antibody,anti-ebs3 antibody, anti-ebs4 antibody,anti-Epidermolysis bullosa simplex antibody, anti-k14 antibody, anti-Keratin 14 antibody,anti-Keratin type I cytoskeletal 14 antibody,anti-Keratin14 antibody, anti-Koebner antibody, anti-Krt 14 antibody, anti-krt14 antibody.
|
|
Specificity
|
This antibody is specific to 50 kD cytokeratin protein designated cytokeratin 14. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified epithelial cells from simple epithelial cells.
|
|
Application Notes
|
Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Cytoplasmic Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)
|
|
Concentration
|
1 to 4 ug/ml
|
|
Buffer
|
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Research Area
|
Cytoskeleton, Cancer
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
|
Publications
|
Purkis, Steel, Mackenzie et al.: "Antibody markers of basal cells in complex epithelia." in: Journal of cell science, Vol. 97 ( Pt 1), pp. 39-50, 1991 (PubMed).
Wetzels, Holland, van Haelst et al.: "Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast." in: The American journal of pathology, Vol. 134, Issue 3, pp. 571-9, 1989 (PubMed).
|
Alternatives for antigen "Keratin 14 (KRT14)", type "Antibodies"
|
Hosts
|
Rabbit (21), Mouse (20), Guinea Pig (2)
|
|
Reactivities
|
Human (40), Rat (Rattus) (7), Mouse (Murine) (4), Pig (Porcine) (3), Dog (Canine) (2), Cat (Feline) (1), Cow (Bovine) (1)
|
|
Applications
|
Western Blotting (WB) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (IHC) (12), Flow Cytometry (FACS) (11), Immunofluorescence (IF) (6), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunoprecipitation (IP) (1)
|
|
Conjugates
|
FITC (1)
|
|
Epitopes
|
C-Term (2), N-Term (2), Center (1)
|
Alternatives 
