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Antibodies
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anti-Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation...
Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (ERCC1) antibody
| Antigen |
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| Synonyms |
UV20, COFS4, Ercc-1, ercc1, MGC53204, ERCC1, MGC140762 |
| Clonality |
Monoclonal (8F1) |
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Host
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Reactivity
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Human (96), Rat (Rattus) (21), Dog (Canine) (20), Mouse (Murine) (20), Pig (Porcine) (17), Cow (Bovine) (16), Monkey (3), Horse (Equine) (2), Sheep (Ovine) (2), Chicken (1), Rabbit (1)
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Application
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Western Blotting (WB) (83), Flow Cytometry (FACS) (58), Immunofluorescence (IF) (51), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), ELISA (6), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN197720 |
| Quantity |
6 ml (1 to 4 ug/ml) |
| Price |
303.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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ERCC1
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Immunogen
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BALB/C mice were injected with full length recombinant human ERCC1 protein.
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Format
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Liquid (prediluted)
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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8F1
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Specificity
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This antibody reacts with a 33-36 KDa protein known as ERCC1 (Excession Repair Cross Complementing) polypeptide. ERCC1 is required for nucleotide excision repair of damaged DNA and is homologous to RAD10. In mammalian cells, XPG cleaves 3
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections using 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (ERCC1)", type "Antibodies"
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Hosts
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Mouse (75), Rabbit (37), Goat (3)
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Reactivities
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Human (96), Rat (Rattus) (21), Dog (Canine) (20), Mouse (Murine) (20), Pig (Porcine) (17), Cow (Bovine) (16), Monkey (3), Horse (Equine) (2), Sheep (Ovine) (2), Chicken (1), Rabbit (1)
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Applications
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Western Blotting (WB) (83), Flow Cytometry (FACS) (58), Immunofluorescence (IF) (51), Immunoprecipitation (IP) (26), Immunohistochemistry (IHC) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), ELISA (6), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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transcript variant 2 (16)
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Alternatives 
