Friend Leukemia Virus Integration 1 (FLI1) antibody

Antigen: Friend Leukemia Virus Integration 1 (FLI1)

Synonyms: EWSR2, SIC-1, FLI1, MGC137447, fli, Xfli, fli1, Xfli1, fli-1, Xfli-1, MGC121593

Clonality: Monoclonal (BV4)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197723

Additional Information

Alternative name: Fli-1

Immunogen: A synthetic cyclic peptides from the antigen

Format: Liquid (prediluted)

Isotype: IgG  (Matching secondary antibodies)

Clone: BV4

Specificity: Fli-1 protein, a member of the ETS family of DNAbinding transcription factors, is involved in cellular proliferation and tumorigenesis. Approximately 90% of Ewing's sarcoma/primitive neuroectodermal tumors (ES/PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein, which can be detected by this antibody. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. Fli-1 has been found to be expressed in the great majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's sarcomas. It has been reported that the high sensitivity and specificity of Fli-1 is equal or exceed those of the established vascular markers, CD31, CD34, and Factor VIII. As the first nuclear, rather than cytoplasmic or membranous marker of endothelium, Fli-1 immunostaining also generally lacks cytoplasmic staining artifacts that are the result of endogenous peroxidases or biotin.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Angiosarcoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Folpe, Hill, Parham et al.: "Immunohistochemical detection of FLI-1 protein expression: a study of 132 round cell tumors with emphasis on CD99-positive mimics of Ewing's sarcoma/primitive neuroectodermal tumor." in: The American journal of surgical pathology, Vol. 24, Issue 12, pp. 1657-62, 2000 (PubMed).

Mhawech-Fauceglia, Herrmann, Penetrante et al.: "Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies." in: Histopathology, Vol. 49, Issue 6, pp. 569-75, 2006 (PubMed).