MSH6 antibody

Antigen: MSH6

Clonality: Monoclonal (44)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197728

Additional Information

Alternative name: MSH 6

Immunogen: Synthetic human MSH6 peptide.

Format: Liquid (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 44

Specificity: MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. This antibody cross reacts with mouse, rat and dog.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil, colon carcinoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Schleger, Heck, Niketeghad et al.: "Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers." in: Experimental cell research, Vol. 236, Issue 2, pp. 418-26, 1997 (PubMed).

Fasano, Theise, Nalesnik et al.: "Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 11, Issue 10, pp. 934-8, 1998 (PubMed).