P63 antibody

Antigen: P63

Clonality: Monoclonal (4A4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197730

Additional Information

Immunogen: A recombinant protein derived from amino acid 1-205 of human p63.

Format: Liquid (prediluted)

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: 4A4

Specificity: This antibody recognizes a 63 kD protein. The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Yang, Kaghad, Wang et al.: "p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities." in: Molecular cell, Vol. 2, Issue 3, pp. 305-16, 1998 (PubMed).

Yang, Schweitzer, Sun et al.: "p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development." in: Nature, Vol. 398, Issue 6729, pp. 714-8, 1999 (PubMed).