Progesterone Receptor (PGR) antibody

Antigen: Progesterone Receptor (PGR)

Synonyms: PR, NR3C3, PR-A, PR-B, BB114106, 9930019P03Rik, ENSMUSG00000074510, PGR, pgr-A, XPR-1, LOC443444, pg, pr, gb:dq017620

Clonality: Monoclonal (BV7)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197732

Additional Information

Alternative name: Progesterone Receptor

Immunogen: A synthetic cyclic peptides from the antigen

Format: Liquid (prediluted)

Isotype: IgG  (Matching secondary antibodies)

Clone: BV7

Specificity: The progesterone receptor is an intracellular steroid receptor that specifically binds progesterone. Expressed by a single gene (chromosome 11q22), it has two main forms, A and B, that differ in their molecular weight. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PR determination provides supplementary information to ER, in predicting response to endocrine therapy as well as estimating survival. PR has proved superior to ER as a prognostic indicator in some studies.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Feil, Clarke, Satyaswaroop: "Progestin-mediated changes in progesterone receptor forms in the normal human endometrium." in: Endocrinology, Vol. 123, Issue 5, pp. 2506-13, 1988 (PubMed).

Clarke, Zaino, Feil et al.: "Monoclonal antibodies to human progesterone receptor: characterization by biochemical and immunohistochemical techniques." in: Endocrinology, Vol. 121, Issue 3, pp. 1123-32, 1987 (PubMed).

Tesch, Shawwa, Henderson: "Immunohistochemical determination of estrogen and progesterone receptor status in breast cancer." in: American journal of clinical pathology, Vol. 99, Issue 1, pp. 8-12, 1993 (PubMed).