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Antibodies
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anti-tyrosinase (Oculocutaneous Albinism IA) (TYR) antibody
tyrosinase (Oculocutaneous Albinism IA) (TYR) antibody
| Antigen |
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| Synonyms |
OCA1A, OCAIA, SHEP3, c, skc35, albino, sandy, zgc:109705, Tyr, C, TYR, tyr, TYRO, LOC100136546 |
| Clonality |
Monoclonal (T311) |
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Host
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Reactivity
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Application
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Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (7), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (5), ELISA (Detection) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN197734 |
| Quantity |
6 ml (1 to 4 ug/ml) |
| Price |
242.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Tyrosinase (Melanoma Marker)
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Immunogen
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BALB/C mice were injected with a recombinant tyrosinase protein.
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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T311
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Specificity
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This antibody is specific to a cluster of proteins between 70-80 kD known as tyrosinase. This antibody does not show any cross-reaction with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas with this antibody showed tyrosinase in melanotic as well as amelanotic variants.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic
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Concentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "tyrosinase (Oculocutaneous Albinism IA) (TYR)", type "Antibodies"
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Hosts
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Rabbit (36), Mouse (10)
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Reactivities
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Human (43), Mouse (Murine) (23), Rat (Rattus) (21), Dog (Canine) (16), Cow (Bovine) (15), Pig (Porcine) (15), Sheep (Ovine) (15), Cat (Feline) (1)
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Applications
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Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (7), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (5), ELISA (Detection) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), AA 1-433 (1)
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Alternatives 
