zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Clonality: Monoclonal (2F3-2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197735

Additional Information

Alternative name: ZAP-70

Immunogen: GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.

Format: Liquid (prediluted)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 2F3-2

Specificity: Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Recent studies suggest that protein levels of ZAP-70 are elevated in B-cells of CLL patients with non-mutant heavy-chain variable region (IgVH) but not those with the mutant regions. Recent evidence suggests that ZAP-70 could be an excellent prognostic biomarker with high levels of the proteins indicating a poor prognosis.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Silvin, Belisle, Abo: "A role for Wiskott-Aldrich syndrome protein in T-cell receptor-mediated transcriptional activation independent of actin polymerization." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21450-7, 2001 (PubMed).

Van Bockstaele, Janssens, Piette et al.: "Kolmogorov-Smirnov statistical test for analysis of ZAP-70 expression in B-CLL, compared with quantitative PCR and IgV(H) mutation status." in: Cytometry. Part B, Clinical cytometry, Vol. 70, Issue 4, pp. 302-8, 2006 (PubMed).