»
Antibodies
»
anti-alpha-Fetoprotein (AFP) antibody
alpha-Fetoprotein (AFP) antibody
| Antigen |
|
| Synonyms |
FETA, HPAFP, AFP, MGC128267 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
|
|
Application
|
Western Blotting (WB) (83), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (55), ELISA (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (20), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
|
| Catalog no. |
ABIN197737 |
| Quantity |
6 ml (1 to 4 ug/ml) (Variants) |
| Price |
135.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Alpha-1-Fetoprotein (AFP)
|
|
Immunogen
|
Alpha-1-fetoprotein isolated from human cord serum.
|
|
Format
|
Liquid (prediluted)
|
|
Specificity
|
This antibody reacts with alpha-1-fetoprotein. A positive staining in the hepatocytes of fetal tissue was observed. This antibody does not react with any other cell or tissue types.
|
|
Application Notes
|
Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Fetal liver. Cellular localization: Cytoplasmic
|
|
Concentration
|
1 to 4 ug/ml
|
|
Buffer
|
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
|
|
Storage
|
Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
|
|
Restrictions
|
For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
|
|
Publications
|
MacDonald, Kelly: "The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 87, Issue 3, pp. 367-72, 1978 (PubMed).
|
Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
|
Hosts
|
Mouse (146), Rabbit (82), Goat (14), Sheep (3), Duck (2)
|
|
Reactivities
|
Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
|
|
Applications
|
Western Blotting (WB) (83), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (55), ELISA (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (20), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Capture) (5), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
|
|
Conjugates
|
HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
|
|
Epitopes
|
N-Term (3), a (2), Center (1), b (1), c (1)
|