Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN197751

Additional Information

Alternative name: Ki-67 Antigen

Immunogen: Synthetic peptide from 62 base pair region of the human Ki-67 antigen.

Format: Liquid (prediluted)

Specificity: This antibody reacts with a nuclear antigen present in proliferating human cells. This antibody cross-reacts with a range of mammalian species. It can be used to evaluate the ki- 67 labeling index in various tumors.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin fixed paraffin embedded tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Wintzer, Zipfel, Schulte-Moenting et al.: "Ki-67 immunostaining in human breast tumors and its relationship to prognosis." in: Cancer, Vol. 67, Issue 2, pp. 421-8, 1991 (PubMed).

Gerdes, Li, Schlueter et al.: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Franco, Chiesa, Beloti et al.: "Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization." in: Journal of biomedical materials research. Part A, Vol. 88, Issue 4, pp. 841-8, 2009 (PubMed).